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Local Humoral Adaptive Immune Response influence on the occurrence of Infertility in Women Infertile Couple with Non-Specific vaginitis due to cross reaction between Staphylococcus aureus and spermatozoa

Anas, Muhammad and Aulani'am, Aulani'am and Wiyasa, I Wayan Arsana and Sardjono, Teguh Wahju and Prawiro, Sumarno Reto (2016) Local Humoral Adaptive Immune Response influence on the occurrence of Infertility in Women Infertile Couple with Non-Specific vaginitis due to cross reaction between Staphylococcus aureus and spermatozoa. In: Temu Ilmiah Nasional dan Rapat Kerja KIBI (Konsorsium Ilmu Biomedik Indonesia), 26-27 Nopember 2016, Grand Clarion Hotel Makassar.

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              Abstract

              The prevalence of infertile couples varies between 10-40%. In Indonesia 17%, East Java 26%, Surabaya 25%, Malang 18% and Mojokerto 25%. Men factors hand of approximately 40%, the rest female factors. The cause of idiopathic infertility approximately 10-30% and allegedly much related to immunological disorders and previous infections. Non-specific vaginitis (VNS) occurring at women infertile couples due to polymicrobial. Staphylococcus aureus (S. aureus) contributes by 21%. In research biocomputation obtained five main protein homolog between protein of human spermatozoa and protein of S. aureus. Homologous sequences obtained by 26-44% and homologous structures ranging from 0.314, 0.332, 4.99, 6.16, and 26.40 Å. Analysis of antigenicity and epitope's expression confirmed that they allow it to be recognized by the same antibodies. Invitro study with 2 isolates of S. aureus which isolate from fertile female partner and infertile female partner. The humoral adaptive immune response occurs in fertile and infertile female partner experiencing VNS due to S. aureus. The molecular weight (MW) of OMP of S. aureus of fertile isolates that was recognized by the s-IgA mucus of the uterine cervix of fertile female partner was 52 kDa while the OMP of S. aureus of infertile isolates was recognized by the s-IgA mucus of the uterine cervix of infertile female partner was 49 kDa. Both of these proteins immunized into mice so that the resulting IgG52 and IgG49. Obtained cross reaction between OMP of S. aureus MW 52 kDa and 49 kDa to OMP of human sperm. MW of human sperm OMP that recognized by IgG52 is 63, 56, 51, 45, and 43 kDa and recognized by IgG49 is 58, 57, 50, 45, 43, 37, 33, 31, 27, and 23 kDa. OMP of human sperm that cross-reacting with OMP of S. aureus is located in the head, neck and tail sperm. Magnitude response of cross reaction between OMP of human sperm with IgG52 significantly different with IgG49, p = 0.000. The magnitude of the response of cross reactions depends on titer IgG52 and IgG49. As conclusion that VNS due to S. aureus may trigger an immune response with s-IgA antibodies produced cross-reacting with OMP of human sperm and the response is depending on the amount of dose. Keywords: infertility, VNS, uterine cervix, S. aureus, OMP, s-IgA, sperm.

              Item Type: Conference or Workshop Item (Speech)
              Subjects: R Medicine > RG Gynecology and obstetrics
              Divisions: Prosiding > Fakultas Kedokteran
              Depositing User: MUHAMMAD ANAS ANAS
              Date Deposited: 25 Feb 2019 13:44
              Last Modified: 28 Feb 2019 12:42
              URI: http://repository.um-surabaya.ac.id/id/eprint/2884

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