Ratnasari , Neneng and Rezkitha, Yudith Annisa Ayu and Adnyana, I Ketut and Alfaray, Ricky Indra and Fauzia , Kartika Afrida and Doohan, Dalla and Panjaitan , Ariel and Priskila , Yobella and Yulinah , Elin and Khomsan, Ali and Waskito, Langgeng Agung and Priyantini , Diah and Syam , Ari Fahrial and Yamaoka, Yoshio and Miftahussurur, Muhammad
(2020)
Anti-Helicobacter pylori Effects of Propolis Ethanol Extract
on Clarithromycin and Metronidazole Resistant Strains.
Systematic Reviews in Pharmacy, 11 (3).
429- 434.
ISSN 0975-8453, 0976-2779
Abstract
Antimicrobial resistance for Helicobacter pylori infection still become
problem in Indonesia especially for metronidazole and clarithromycin
resistance that is commonly used in the national regiment therapy.
Finding a new antimicrobial agent against this bacterium is still a
challenge to face. Propolis extract is well known substance which has
antimicrobial effect against some gram-negative bacteria. However, the
effect against H. pylori remain unknown. We analysed the antimicrobial
effect of propolis extract against H. pylori in clarithromycin and
metronidazole Resistant Strains. Anti-H. pylori effect of ethanol propolis
extract was tested on 10 types of H. pylori strains isolated from
dyspeptic patients in Indonesia. Ethanol propolis extracts (PRO) show
inhibitory activity at concentrations of 5% and 10% on all strains H. pylori.
Determination minimum inhibitory concentration (MIC) value of propolis
extract used microdilution method, the MIC values were in the range of
1024-8192 µg/mL. Determination of the minimum bactericidal
concentration (MBC) value is performed by scratch method based on the
results of the microdilution test and produces an MBC value in the range of 1024 - 16348 µg/mL. The required concentration of propolis extract
for the bactericidal effect against H. pylori was the same or eight-fold
higher than the corresponding value. The combination of propolis extract
and metronidazole based through paper tape method showed an
additive effect, as well as the combination of propolis extract and
clarithromycin. This extract might be can become alternative treatment
against H. pylori infection that resistance for metronidazole and
clarithromycin treatment
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