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Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates

Fauzia, Kartika Afrida and Miftahussurur, Muhammad and Syam, Ari Fahrial and Waskito, Langgeng Agung and Doohan, Dalla and Rezkitha, Yudith Annisa Ayu and Matsumoto, Takashi and Tuan, Vo Phuoc and Akada, Junko and Yonezawa, Hideo and Kamiya, Shigeru and Yamaoka, Yoshio (2020) Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates. Toxins, 12 (8). pp. 1-14. ISSN 2072-6651

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Abstract: We evaluated biofilm formation of clinical Helicobacter pylori isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to measure the planktonic susceptibility of 101 H. pylori strains. Biofilms were quantified by the crystal violet method. The minimum biofilm eradication concentration (MBEC) was obtained by measuring the survival of bacteria in a biofilm after exposure to antibiotics. The majority of the strains formed a biofilm (93.1% (94/101)), including weak (75.5%) and strong (24.5%) biofilm-formers. Planktonic resistant and sensitive strains produced relatively equal amounts of biofilms. The resistance proportion, shown by theMBECmeasurement,washigherinthestrongbiofilmgroupforallantibioticscomparedtotheweak biofilm group, especially for clarithromycin (p = 0.002). Several cases showed sensitivity by the MIC measurement, but resistance according to the MBEC measurements (amoxicillin, 47.6%; tetracycline, 57.1%; clarithromycin, 19.0%; levofloxacin, 38.1%; and metronidazole 38.1%). Thus, biofilm formation may increase the survival of H. pylori and its resistance to antibiotics. Biofilm-related antibiotic resistance should be evaluated with antibiotic susceptibility.

Item Type: Article
Uncontrolled Keywords: Helicobacter pylori; antibiotic resistance; biofilm formation; biofilm-specific resistance; amoxicillin; clarithromycin; levofloxacin; metronidazole; tetracycline
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Jurnal > Fakultas Kedokteran
Date Deposited: 14 Aug 2020 13:54
Last Modified: 18 Nov 2020 09:57

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