The potency of bacteria isolated from the hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and indole acetic acid (IAA) production

Arimurti, Anindita Riesti Retno and Rohmayani, Vella and Romadhon, Nurhidayatullah (2022) The potency of bacteria isolated from the hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and indole acetic acid (IAA) production. Biogenesis Jurnal Ilmiah Biologi, 10 (1). pp. 112-120. ISSN 2580-2909

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Abstract

Rockwool is a growing media widely used by hydroponic farmers. The hydroponic system contains high amounts of nutrients in order to provide an ideal environment for microorganisms to grow. Microorganisms can help spur plant growth through many mechanisms such as N2 fixation, plant stress control, nutrient extraction from soil, anti-pathogenic functionality, production of various types of plant hormones, and biological control. This study aimed to determine the potential of bacteria in hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and IAA (Indole Acetic Acid) production. The study began with isolating Hydroponic Rockwool Bacteria from Brassica rapa L., followed by identifying and growing isolates on NA, NB, and LB media for 48 hours to determine their spesies. After 48 hours, counting the number of bacteri and characteristics of the colonies. Then the bacterial colonies found were purified on NA media. After knowing their spesies, the potential Nitrogen fixation was tested using NFBB media for six days. Then the potential for IAA production was tested using LB medium for one week. The results showed that 11 (eleven) bacterial isolates could not fixation Nitrogen but had the ability to produce IAA hormone, with IAA concentrations ranging from 4.09 to 21.99 ppm. Keywords: field mustard (Brassica rapa L.); IAA (Indole Acetic Acid); Nitrogen fixation; Rockwool

Item Type:	Article
Subjects:	Q Science > QR Microbiology
Divisions:	Jurnal > Fakultas Ilmu Kesehatan
Depositing User:	ANINDITA RIESTI RETNO ARIMURTI
Date Deposited:	31 Mar 2023 11:19
Last Modified:	31 Mar 2023 11:19
URI:	http://repository.um-surabaya.ac.id/id/eprint/6608

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