COMBINATION POTENTIAL SOAKING TEAK LEAVES (Tectona Grandis) WITH HCl ON STAINING GRAM IN Escherichia coli BACTERIA

Assyahida, Safina and Artanti, Dita and SAMSUDIN, RINZA RAHMAWATI and Sari, Yeti Eka Sispita and Azizah, Fitrotin (2022) COMBINATION POTENTIAL SOAKING TEAK LEAVES (Tectona Grandis) WITH HCl ON STAINING GRAM IN Escherichia coli BACTERIA. Prosiding : International Conference on Medical Laboratory Technology, 2 (1).

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Abstract

Safranin or fuchin is a dye that is widely used in the fields of industry, textiles, histology, cytology and bacteriology. Safranin has a very expensive price in 2019 reaching 3,565,000 / 25 grams. Thus, it is necessary to substitute dyes from natural and much cheaper materials. Teak leaves (Tectona grandis), especially the young ones, contain the pigment pheophitin, ß-carotene, chlorophyll and two other pigments that have not been identified as well as several anthocyanin derivatives, namely, pelargonidin 3-glucoside, pelargonidin 3,7diglucoside. Anthocyanins contained in teak leaf (Tectona grandis) buds are reddish brown. The presence of these pigments can be used in laboratory tests for staining bacteria. The purpose of this study was to determine the soaking variation of teak leaf (Tectona grandis) buds for 24 hours, 48 hours, 72 hours, 96 hours in 96% alcohol with concentrated HCl, and the use of safranin as a positive control. The data of the research was in the form of scores of observations. Based on the Kursal Wallis alpha test, it was found that there was a significant difference in the results of staining bacteria among various soaking of teak leaf (Tectona grandis) buds in 96% alcohol with concentrated HCl in staining gram technique. Soaking teak leaf (Tectona grandis) buds in 96% alcohol with HCl for 72 hours and 96 hours gave the same staining results as safranin. The results showed that soaking teak leaf (Tectona grandis) buds in alcohol with concentrated HCl can be used as an alternative natural dye for the method of staining Gram on Escherichia coli bacteria. Keywords: Safranin, anthocyanin, teak leaf (Tectona Grandis), Gram staining, Eschericia coli

Item Type:	Article
Subjects:	Q Science > QR Microbiology
Divisions:	Prosiding > Fakultas Ilmu Kesehatan
Depositing User:	FITROTIN AZIZAH AZIZAH
Date Deposited:	11 Oct 2023 10:24
Last Modified:	11 Oct 2023 10:24
URI:	http://repository.um-surabaya.ac.id/id/eprint/7341

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