Differences in Response of Staphylococcus aureus Protein to Serum IgG and Secretion of Uterine Cervical s-IgA from Fertile and Infertile Couples Women with Non-Specific Vaginitis

The cause of idiopathic infertility approximately 10–30% and allegedly much related to immunological disorders and previous infections. We explore the response of circular and local humoral adaptive immune system at women of the fertile and infertile couple to infection Staphylococcus aureus (S. aureus) at a lower genital tract of women with nonspecific vaginitis (NSV). Design: Biocomputational and In vitro study. Result: Five S. aureus protein have high homology sequences and structures. Analysis of antigenicity, all of the molecule obtain high antigenicity properties and all of S. aureus protein express their epitope, only 80% molecule of human sperm protein does. So all of the proteins almost confirmed that they allow it to be recognized by the same antibodies. Two isolates of S. aureus from a fertile women partner (S. aureus_fertile) and infertile women partner (S. aureus_infertile) is used to collect its outer membrane protein (OMP). The circular and local humoral adaptive immune response occur in the women of a fertile and infertile couple experiencing NSV due to S. aureus. The molecular weight (MW) of OMP of S. aureus_fertile that was recognized by the s-IgA_fertile was 52 kDa while the OMP of S. aureus_infertile was recognized by the s-IgA_infertile was 49 kDa. The molecular weight (MW) of OMP of S. aureus_fertile that was recognized by the IgG_fertile was 142, 128, 87, 85, 67, 65, 52, 49, 32, 29, 28, and 19 kDa while the OMP of S. aureus_infertile was recognized by the IgG_infertile was 143, 138, 123, 117, 61, 57, 52, 49, 45, 36, 33, 28, 26, 18, 17, 15, 14, and 11 kDa. They obtain cross-reaction between OMP of S. aureus_fertile and OMP of S. aureus_infertile via both IgG and s-IgA. s-IgA antibodies more specific than IgG antibodies.