Characterization of a novel Helicobacter pylori East Asian‑type CagA ELISA for detecting patients infected with various cagA genotypes

Doohan, Dalla  and Miftahussurur, Muhammad  and Matsuo, Yuichi  and Kido, Yasutoshi  and Akada, Junko  and Matsuhisa, Takeshi  and Yee, Than Than  and Htet, Kyaw  and Aftab, Hafeza  and Vilaichone, Ratha‑korn  and Mahachai , Varocha and Ratanachu‑ek, Thawee  and Tshering, Lotay  and Waskito, Langgeng Agung  and Fauzia, Kartika Afrida  and Uchida, Tomohisa  and Syam, Ari Fahrial  and Rezkitha, Yudith Annisa Ayu  and Yamaoka, Yoshio  (2019) Characterization of a novel Helicobacter pylori East Asian‑type CagA ELISA for detecting patients infected with various cagA genotypes. Medical Microbiology and Immunology, 209. pp. 29-40. ISSN 1432-1831, 0300-8584

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Abstract

Currently, Western-type CagA is used in most commercial Helicobacter pylori CagA ELISA kits for CagA detection rather than East Asian-type CagA. We evaluated the ability of the East Asian-type CagA ELISA developed by our group to detect anti-CagA antibody in patients infected with different cagA genotypes of H. pylori from four different countries in South Asia and Southeast Asia. The recombinant CagA protein was expressed and later purified using GST-tag affinity chromatog- raphy. The East Asian-type CagA-immobilized ELISA was used to measure the levels of anti-CagA antibody in 750 serum samples from Bhutan, Indonesia, Myanmar, and Bangladesh. The cutoff value of the serum antibody in each country was determined via Receiver-Operating Characteristic (ROC) analysis. The cutoff values were different among the four countries studied (Bhutan, 18.16 U/mL; Indonesia, 6.01 U/mL; Myanmar, 10.57 U/mL; and Bangladesh, 6.19 U/mL). Our ELISA had better sensitivity, specificity, and accuracy of anti-CagA antibody detection in subjects predominantly infected with East Asian-type CagA H. pylori (Bhutan and Indonesia) than in those infected with Western-type CagA H. pylori predominant (Myanmar and Bangladesh). We found positive correlations between the anti-CagA antibody and antral monocyte infiltra- tion in subjects from all four countries. There was no significant association between bacterial density and the anti-CagA antibody in the antrum or the corpus. The East Asian-type CagA ELISA had improved detection of the anti-CagA antibody in subjects infected with East Asian-type CagA H. pylori. The East Asian-type CagA ELISA should, therefore, be used in populations predominantly infected with East Asian-type CagA.

Item Type:	Article
Uncontrolled Keywords:	East Asian-type CagA · ELISA · Helicobacter pylori · Anti-CagA antibody
Subjects:	Q Science > QR Microbiology R Medicine > R Medicine (General)
Divisions:	Jurnal > Fakultas Kedokteran
Depositing User:	YUDITH ANNISA AYU REZKITHA
Date Deposited:	14 Aug 2020 12:47
Last Modified:	18 Nov 2020 10:42
URI:	http://repository.um-surabaya.ac.id/id/eprint/4225

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