Anas, Muhammad and Utomo, Didik Huswo and Widjajanto, Edi and Wiyasa, I Wayan Arsana and Aulani'am, Aulani'am and Prawiro, Sumarno Reto (2017) Revealing Cross-Reaction Antibody between Staphylococcus aureus and Human Spermatozoa Protein by Biocomputational approach to Discover Infertility Mechanism. In: BIT's 7th Annual World Congress of Molecular & Cell Biology (CMCB-2017), April 25-27, 2017, Hilton Hotel Xi'an China.
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Abstract
Infertile women with non-specific vaginitis due to Staphylococcus aureus (S. aureus) will develop antibodies that cross-react with human spermatozoa, causing infertility. We evaluate the homology of S. aureus and spermatozoa proteins based on sequences and structural model through computational approach. All proteins of human spermatozoa were retrieved from UNIPROT. Modeling protein was constructed based on threading modeling. Sequence homology analysis was evaluated using BLASTP and structural comparison was done by Superpose v.1.0. Antigenicity and epitope mapping of homolog proteins were conducted using IEDB web services for comparing the potential cross-reaction antibody. The result of sequence comparison showed that there are 5 homolog proteins from human spermatozoa and S. aureus. Based on structural analysis, antigenicity and epitope mapping, potential candidate protein that have cross-reaction with human antibody are protein deglycase DJ-1, Sperm acrosome membrane-associated protein 4, UDP-N-acetyl hexosamine pyrophosphorylase. These proteins have similar structure and similar position of epitope that locate on surface protein. In addition the protein have high antigenicity profile. It can be concluded that the similar properties of sequence, structure, antigenicity and epitope from those proteins are possible if human antibody can cross-react with human spermatozoa protein after infecting by S. aureus. This mechanism may have role in infertile woman
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Subjects: | R Medicine > RG Gynecology and obstetrics |
| Divisions: | Prosiding > Fakultas Kedokteran |
| Depositing User: | MUHAMMAD ANAS ANAS |
| Date Deposited: | 11 Jan 2024 03:59 |
| Last Modified: | 11 Jan 2024 03:59 |
| URI: | http://repository.um-surabaya.ac.id/id/eprint/2891 |
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