EFFECT OF CHITOSAN ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS (RATTUS NORVEGICUS) EXPOSED TO LEAD ACETATE

Annisa, Wigati R (2020) EFFECT OF CHITOSAN ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS (RATTUS NORVEGICUS) EXPOSED TO LEAD ACETATE. Institute of Medico-Legal Publications, Indian Journal of Public Health Research & Development,.

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Abstract

Context: Lead exposure can have a harmful effect on the body, one of which is the reproduction system. Lead can increase oxidative stress that can cause damage to organ. This research aims to prove the effect of chitosan in reducing MDA levels and increasing 17β estradiol level and VEGF expression due to lead exposure. This study was a true experimental in vivostudy using apost-test only group control design. This study used female wistar strain ratsas the experimental animals that were divided into anegative control group,a positive control group (dose of lead 175 mg/kg/BW) and treatment groups (dose of chitosan 16, 32, and 64 mg/kg/BW) for 30 days.Surgery was carried out during the proestrus phase. The MDA level measure using a microplate reader, 17β Estradiol level using ELISA, and the VEGF expression using IHC.This study used One Way ANOVA testand LSD test.The dose of chitosan thatwas considered capable of reducing uterine MDA level was treatment group 2 (dose of 32 mg/Kg/BW).Administration of chitosan at dose 3 (dose of 64 mg/Kg/BW) was able to increase Estradiol 17β levels and VEGF expression optimally. Chitosan can quantitatively reduce MDA level and increase 17β estradiol. In addition, chitosan administration can significantly increase VEGF expression. Keywords: Reproduction, Lead, chitosan, Malondialdehyde, 17β Estradiol, VEG

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Laporan Penelitian
Depositing User:	Annisa' Wigati Rozifa
Date Deposited:	21 Mar 2023 08:54
Last Modified:	21 Mar 2023 08:54
URI:	http://repository.um-surabaya.ac.id/id/eprint/6413

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