Perbandingan Indeks Eritrosit pada Rattus norvegicus yang Terpapar Kebisingan dan yang Tidak Terpapar Kebisingan

Azzahra, Amelia (2026) Perbandingan Indeks Eritrosit pada Rattus norvegicus yang Terpapar Kebisingan dan yang Tidak Terpapar Kebisingan. Applied Bachelor thesis, Universitas Muhammadiyah Surabaya.

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Abstract

Latar belakang penelitian ini adalah kebisingan merupakan salah satu bentuk polusi lingkungan yang dapat menimbulkan stres fisiologis dan memengaruhi berbagai sistem tubuh, termasuk sistem hematopoietik. Stres akibat kebisingan dapat memicu perubahan pada eritrosit yang berpotensi memengaruhi nilai Indeks Eritrosit. Tujuan penelitian ini adalah untuk mengetahui perbandingan hasil Indeks Eritrosit yang meliputi Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), dan Mean Corpuscular Hemoglobin Concentration (MCHC) pada Rattus norvegicus yang terpapar dan tidak terpapar kebisingan. Metode penelitian yang digunakan adalah penelitian kuantitatif dengan desain true experimental menggunakan rancangan post test only control group design. Sampel penelitian berupa 16 ekor Rattus norvegicus jantan yang dibagi menjadi kelompok perlakuan dan kelompok kontrol masing-masing sebanyak 8 ekor. Kelompok perlakuan diberikan paparan kebisingan ≥85 dB selama 3 jam/hari selama 5 hari berturut-turut, sedangkan kelompok kontrol tidak diberikan paparan kebisingan. Pemeriksaan Indeks Eritrosit dilakukan menggunakan hematology analyzer berupa alat Sysmex XN 450. Data dianalisis menggunakan uji Shapiro-Wilk dan dilanjutkan dengan uji Independent Sample T-Test. Hasil penelitian menunjukkan bahwa rerata MCV pada kelompok perlakuan sebesar 58,2 ± 4,3 fL dan kelompok kontrol 56,7 ± 3,8 fL, rerata MCH sebesar 17,5 ± 0,6 pg dan 17,3 ± 1,0 pg, serta rerata MCHC sebesar 29,9 ± 1,3 g/dL dan 30,3 ± 1,5 g/dL. Hasil uji Independent Sample T-Test menunjukkan nilai signifikansi MCV (p=0,42), MCH (p=0,57), dan MCHC (p=0,52), sehingga seluruh parameter memiliki nilai p > 0,05. Kesimpulan penelitian ini adalah tidak terdapat perbedaan yang signifikan pada Indeks Eritrosit (MCV, MCH, dan MCHC) antara Rattus norvegicus yang terpapar kebisingan dan yang tidak terpapar kebisingan.

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The background of this research is that noise is a form of environmental pollution that can cause physiological stress and affect various body systems, including the hematopoietic system. Stress-induced noise can trigger changes in erythrocytes that may affect Erythrocyte Index values. This study aims to determine the comparison of Erythrocyte Index results, which include Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), and Mean Corpuscular Hemoglobin Concentration (MCHC) in Rattus norvegicus exposed and not exposed to noise. The research method used is quantitative, with a true experimental design and a post-test-only control group. The research sample consisted of 16 male Rattus norvegicus, divided into treatment and control groups, each with 8 individuals. The treatment group was exposed to noise ≥85 dB for 3 hours/day for 5 consecutive days, while the control group was not exposed to noise. Erythrocyte Index examination was performed using a hematology analyzer, specifically the Sysmex XN-450. Data were analyzed using the Shapiro-Wilk test followed by the Independent Sample T-Test. The research results showed that the mean MCV in the treatment group was 58.2 ± 4.3 fL, and in the control group was 56.7 ± 3.8 fL; the mean MCH was 17.5 ± 0.6 pg and 17.3 ± 1.0 pg; and the mean MCHC was 29.9 ± 1.3 g/dL and 30.3 ± 1.5 g/dL. The results of the Independent Sample T-Test showed non-significant p-values for MCV (p=0.42), MCH (p=0.57), and MCHC (p=0.52), indicating that all parameters had p-values> 0.05. This study concludes that there is no significant difference in Erythrocyte Indices (MCV, MCH, and MCHC) between Rattus norvegicus exposed to noise and those not exposed to noise.

Item Type: Thesis (Applied Bachelor)
Uncontrolled Keywords: Kebisingan, Indeks Eritrosit, MCV, MCH, MCHC, Rattus norvegicus, Noise, Erythrocyte Index, MCV, MCH, MCHC, Rattus norvegicus
Subjects: Q Science > QL Zoology
Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RB Pathology
Divisions: 04. Fakultas Ilmu Kesehatan > D4 Ahli Teknologi Laboratorium Medis
Depositing User: Amelia Azzahra
Date Deposited: 08 Sep 2026 06:10
Last Modified: 08 Sep 2026 06:10
URI: https://repository.um-surabaya.ac.id/id/eprint/13883

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