Daya Antibakteri Kamandin Saebo (Glossocardia leschenaultii) terhadap Bakteri Staphylococcus aureus sebagai Sumber Belajar Masyarakat dalam Bentuk E-Booklet

Penelitian ini bertujuan untuk mengetahui Daya Antibakteri Kamandin Saebo (Glossocardia leschenaultii) Terhadap Bakteri Staphylococcus aureus serta mengembangkan Hasil penelitian sebagai Sumber Belajar dalam Bentuk E-booklet. Jenis penelitian berupa penelitian kuantitatif. Penelitian ini menggunakan metode eksperimental dengan desain Rancangan Acak Lengkap yang terdiri atas empat perlakuan konsentrasi ekstrak, yaitu 100%, 75%, 50%, 25%, 0% (kontrol negatif DMSO) serta kontrol positif cotrimoxazole. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram (Kirby-Bauer). Hasil Penelitian menunjukkan semua perlakuan ekstrak menunjukkan perbedaan signifikan dibandingkan dengan P5 (0%) dan P6 (kontrol positif Cotrimoxazole) (p < 0,05). Sementara itu, Konsentrasi Ekstrak Kamandin Saebo (Glossocardia leschenaultii) P1 (100%), (P2) 75%, (P3) 50%, dan (P5) 25% menunjukkan aktivitas antibakteri yang sama terhadap pertumbuhan bakteri Staphylococcus aureus. Rata-rata diameter zona hambat pada konsentrasi P1 (100%), (P2) 75%, (P3) 50%, dan (P5) 25% berturut-turut sebesar 15,2 mm, 16,1 mm, 16,9 mm, dan 11,8 mm. Berdasarkan nilai rata-rata semua konsentrasi ekstrak termasuk dalam Kategori Kuat. Aktivitas antibakteri tersebut diduga berkaitan dengan kandungan senyawa metabolit sekunder yang terdapat pada Kamandin Saebo, seperti flavonoid, alkaloid, tanin, dan steroid. Hasil penelitian berupa e-booklet dan dapat diakses melalui website flipbook Heyzine.
Kata Kunci: Antibakteri, Kamandin Saebo, Staphylococcus aureus, E-booklet.

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ABSTRACT

Sakinah, Hanunah Dina. 2026. The Antibacterial Activity of Kamandin Saebo (Glossocardia leschenaultii) Against Staphylococcus aureus as an community Educational Resource in the Form of an E-booklet. Faculty of Education, Communication, and Science, Muhammadiyah University of Surabaya. Advisor I: Dr. Peni Suharti, M.Kes., Advisor II: Dr. Lina Listiana, M.Kes.
This study aims to evaluate the antibacterial activity of Kamandin Saebo (Glossocardia leschenaultii) extract against Staphylococcus aureus and to develop the research findings into a community learning resource in the form of an e-booklet. This study employed a quantitative experimental approach using a a Completely Randomized Design (CRD). The experiment consisted of four extract concentrations (100%, 75%, 50%, and 25%), a negative control (0% DMSO), and a positive control (cotrimoxazole). Antibacterial activity was evaluated using the Kirby–Bauer disk diffusion method. The results showed that all extract treatments differed significantly from the negative control (0% DMSO) and the positive control (cotrimoxazole) (p < 0.05). The extract concentrations of 100%, 75%, 50%, and 25% exhibited comparable antibacterial activity against the growth of Staphylococcus aureus, with mean inhibition zone diameters of 15.2 mm, 16.1 mm, 16.9 mm, and 11.8 mm, respectively. Based on the inhibition zone classification, all extract concentrations demonstrated strong antibacterial activity. This activity is likely attributable to the presence of secondary metabolites, including flavonoids, alkaloids, tannins, and steroids. The findings of this study were subsequently developed into an e-booklet as a community learning resource and are accessible through the Heyzine Flipbook platform.
Keywords : antibacterial activity; Glossocardia leschenaultii; Staphylococcus aureus; e-booklet; community learning resource