Damayanti, Nimas Fatihah Intan (2026) Pengaruh Hasil Pewarnaan Hematoksilin Eosin Pada Sistem Respirasi Rattus norvegicus Yang Terpapar CO2 Dengan Terapi Murottal. Applied Bachelor thesis, Universitas Muhammadiyah Surabaya.
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Abstract
Paparan karbon dioksida (CO₂) dapat menyebabkan stres oksidatif dan kerusakan jaringan paru yang memengaruhi kualitas preparat histologi, terutama kejelasan inti sel dan sitoplasma pada pewarnaan Hematoksilin Eosin. Terapi murottal Al-Qur'an diduga memiliki efek relaksasi yang dapat membantu mempertahankan integritas jaringan. Tujuan penelitian ini untuk mengetahui pengaruh terapi murottal terhadap kualitas hasil pewarnaan Hematoksilin Eosin pada sistem respirasi Rattus norvegicus yang terpapar CO₂. Metode penelitian ini menggunakan eksperimental dengan 14 ekor Rattus norvegicus yang dibagi menjadi enam kelompok, yaitu satu kelompok kontrol dan lima kelompok perlakuan yang diberikan terapi murottal dengan frekuensi berbeda. Penilaian dilakukan berdasarkan kejelasan inti sel dan sitoplasma menggunakan sistem skor, kemudian dianalisis dengan uji Shapiro-Wilk dan Kruskal-Wallis. Hasil penelitian menunjukkan bahwa kelompok kontrol memperoleh skor 1 (kurang jelas), sedangkan kelompok perlakuan menunjukkan peningkatan kualitas preparat dengan skor 2 (jelas) hingga skor 3 (sangat jelas). Hasil uji Kruskal-Wallis menunjukkan nilai signifikansi 0,123 (p>0,05), sehingga tidak terdapat pengaruh yang signifikan secara statistik. Kesimpulan penelitian ini adalah Pemberian terapi murottal menunjukkan kualitas hasil pewarnaan Hematoksilin Eosin yang lebih baik pada sistem respirasi Rattus norvegicus yang terpapar CO₂ dibandingkan kelompok kontrol tanpa terapi murottal. Kelompok perlakuan memperoleh skor 2 (jelas) hingga skor 3 (sangat jelas), sedangkan kelompok kontrol memperoleh skor 1 (kurang jelas), yang menunjukkan kejelasan inti sel dan sitoplasma paling rendah.
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Carbon dioxide (CO₂) exposure can cause oxidative stress and lung tissue damage, thereby affecting the quality of histological preparations, particularly the clarity of cell nuclei and cytoplasm in Hematoxylin-Eosin staining. Al-Qur'an murottal therapy is suspected to have a relaxing effect that can help maintain tissue integrity. This study aims to determine the effect of murottal therapy on the quality of Hematoxylin-Eosin staining in the respiratory system of Rattus norvegicus exposed to CO₂. The research method used is experimental, with 14 Rattus norvegicus divided into six groups: one control group and five treatment groups that received murottal therapy at different frequencies. The assessment was conducted using a scoring system based on the clarity of the cell nucleus and cytoplasm, and the results were analyzed with the Shapiro-Wilk and Kruskal-Wallis tests. The research results showed that the control group received a score of 1 (less clear), whereas the treatment groups showed improved preparation quality, with scores ranging from 2 (clear) to 3 (very clear). The Kruskal-Wallis test results indicated a significance value of 0.123 (p>0.05); thus, there was no statistically significant effect. This study concludes that administration of murottal therapy yields higher-quality Hematoxylin-Eosin staining results in the respiratory system of Rattus norvegicus exposed to CO₂, compared with the control group without murottal therapy. The treatment group received scores ranging from 2 (clear) to 3 (very clear), while the control group received a score of 1 (less clear), indicating the lowest clarity of cell nuclei and cytoplasm.
| Item Type: | Thesis (Applied Bachelor) |
|---|---|
| Uncontrolled Keywords: | Hematoksilin Eosin, murottal, CO₂, Rattus norvegicus, kejelasan inti sel dan sitoplasma, Hematoxylin Eosin, murottal, CO₂, Rattus norvegicus, clarity of cell nuclei and cytoplasm |
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RB Pathology |
| Divisions: | 04. Fakultas Ilmu Kesehatan > STr. Teknologi Laboratorium Medis |
| Depositing User: | NIMAS FATIHAH INTAN DAMAYANTI |
| Date Deposited: | 17 Oct 2026 05:46 |
| Last Modified: | 17 Oct 2026 05:51 |
| URI: | https://repository.um-surabaya.ac.id/id/eprint/14161 |
