Kualitas Hasil Pewarnaan Hematoxylin Eosin pada Jaringan Sistem Pencernaan Rattus norvegicus yang Terpapar Murottal

Latar Belakang: Paparan stres dapat mempengaruhi sistem pencernaan, khususnya lambung, melalui peningkatan aktivitas sistem saraf simpatis dan sekresi hormon stres yang berpotensi menyebabkan perubahan struktur jaringan lambung. Murottal Al-Qur'an merupakan salah satu terapi nonfarmakologis yang diketahui dapat memberikan efek relaksasi dan menurunkan respons stres. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh variasi waktu paparan murottal terhadap gambaran histologi lambung Rattus norvegicus yang terpapar CO₂. Metode: Penelitian ini merupakan penelitian eksperimental dengan rancangan post-test only control group design. Hewan uji dibagi menjadi kelompok kontrol dan kelompok perlakuan yang diberikan paparan murottal sebanyak 1 kali, 2 kali, 3 kali, 4 kali, dan 5 kali per hari selama 10 menit. Jaringan lambung dibuat preparat histologi menggunakan pewarnaan Hematoxylin-Eosin dan diamati secara mikroskopis. Analisis data dilakukan menggunakan uji normalitas Shapiro-Wilk, uji homogenitas Levene, dan uji One Way ANOVA. Hasil: Hasil pengamatan menunjukkan bahwa gambaran histologi lambung pada seluruh kelompok relatif serupa. Struktur lambung masih dapat diamati dengan inti sel berwarna biru keunguan dan sitoplasma berwarna merah muda. Beberapa preparat pada kelompok dengan intensitas paparan murottal yang lebih tinggi menunjukkan struktur jaringan yang lebih jelas, namun tidak ditemukan secara konsisten pada seluruh sampel. Hasil analisis statistik menunjukkan nilai signifikansi uji normalitas sebesar 0,006, uji homogenitas sebesar 0,385, dan uji Kruskal Wallis sebesar 0,094 (p > 0,05), sehingga tidak terdapat perbedaan yang signifikan antar kelompok perlakuan. Kesimpulan: Variasi waktu paparan murottal tidak memberikan pengaruh yang signifikan terhadap gambaran histologi lambung Rattus norvegicus yang terpapar CO₂. Meskipun frekuensi paparan yang lebih tinggi cenderung menghasilkan gambaran jaringan yang lebih jelas pada beberapa preparat, perbedaan tersebut tidak bermakna secara statistik.
Kata Kunci: murottal Al-Qur'an, histologi lambung, Rattus norvegicus, stres, paparan CO₂.

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Background: Exposure to stress can affect the digestive system, particularly the stomach, through increased activity of the sympathetic nervous system and the secretion of stress hormones, which can potentially cause changes in the structure of stomach tissue. Recitation of the Qur’an is a non-pharmacological therapy known to have a relaxing effect and to reduce the stress response. Objective: This study aimed to determine the effect of varying durations of murottal exposure on the histological profile of the stomach in Rattus norvegicus exposed to CO₂. Methods: This was an experimental study using a post-test only control group design. Test animals were divided into a control group and treatment groups that were exposed to the recitation of the Qur’an 1, 2, 3, 4, and 5 times per day for 10 minutes. Gastric tissue was prepared for histological examination using Hematoxylin-Eosin staining and observed under a microscope. Data analysis was performed using the Shapiro-Wilk normality test, Levene’s homogeneity test, and one-way ANOVA. Results: The observations showed that the histological appearance of the stomach was relatively similar across all groups. The structure of the stomach was still observable, with cell nuclei appearing bluish-purple and cytoplasm appearing pink. Some specimens in the groups with higher murottal exposure intensity showed a clearer tissue structure, though this was not consistently observed across all samples. The results of the statistical analysis showed a significance value of 0.006 for the normality test, 0.385 for the homogeneity test, and 0.094 for the Kruskal-Wallis test (p > 0.05), indicating that there were no significant differences among the treatment groups. Conclusion: Variations in the duration of murottal exposure did not have a significant effect on the histological appearance of the stomachs of CO₂-exposed Rattus norvegicus. Although higher exposure intensity tended to produce a clearer tissue pattern in some specimens, these differences were not statistically significant.
Keywords: Murottal Al-Qur’an, gastric histology, Rattus norvegicus, stress, CO₂ exposure.