The Potential of Indigenous Bacteria from Various Soil Samples in Degrading Polymer Plastic Waste

Anindita Riesti Retno Arimurti, S.Si., M.Si and Vella Rohmayani, S.Pd., M.Si and Nurhidayatullah Romadhon, S.Pd., M.Si (2023) The Potential of Indigenous Bacteria from Various Soil Samples in Degrading Polymer Plastic Waste. Al-Hayat: Journal of Biology and Applied Biology, 6 (2). pp. 111-125. ISSN 2622-0725

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Abstract

The accumulation of plastic waste is a significant global issue encountered by many countries. Due to its resistance to natural degradation, plastic waste eventually finds its way into the soil, leading to alterations in soil composition. Plastic, a complex polymer with diverse applications, has become integral to various aspects of life. Correspondingly, the extensive use of High-Density Polyethylene (HDPE) and Low-Density Polyethylene (LDPE) plastics has escalated waste production. Hence, this research aimed to isolate indigenous bacteria from diverse soil samples obtained from Surabaya's landfill area and mangrove forests, specifically focusing on their capability to degrade HDPE and LDPE plastic polymers. Sampling was conducted at the Keputih bamboo forest in Surabaya and the Final Disposal Site (TPA). Bacteria were subsequently separated from the plastic waste and soil samples. Afterward, the isolated bacteria were purified and screened for their ability to degrade LDPE and HDPE polymers. The isolation process yielded 19 isolates, predominantly bacilli, Gramnegative, and catalase-positive bacteria capable of fermenting lactose and glucose, with cell sizes ranging from 1 to 5 µm. Screening tests revealed that Isolate 17 (K2D3U1 MWJ), originating from the Wonorejo mangrove forest, was identified as Pseudomonas sp. with an LDPE degradation ability of 21.64%. On the other hand, Isolate 2 (K1D2U2 KPH), obtained from the Keputih Final Disposal Site (TPA), was identified as Bacillus sp. with an HDPE degradation ability of 27.13%.

Item Type:	Article
Subjects:	R Medicine > R Medicine (General)
Divisions:	Modul > Fakultas Hukum
Depositing User:	Vella Yani Vella
Date Deposited:	04 Dec 2024 06:05
Last Modified:	04 Dec 2024 06:05
URI:	http://repository.um-surabaya.ac.id/id/eprint/9677

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