PHYTOCHEMISTRY SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN KARO TRADITIONAL OIL, AN INDONESIAN TRADITIONAL HERBAL MEDICINE

Primadina, Nova (2020) PHYTOCHEMISTRY SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN KARO TRADITIONAL OIL, AN INDONESIAN TRADITIONAL HERBAL MEDICINE. Asian Journal of pharmaceutical and clinical Research (AJPCR), 13 (2). pp. 204-208. ISSN (Online ISSN: 2455-3891 | Print ISSN: 0974-2441)

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Abstract

Objective: The purpose of this research was to observe the bioactive compounds of Karo traditional oil by phytochemistry screening and gas chromatography-mass spectrometry (GC-MS) analysis. Method: We use 150 mg sample of traditional Karo traditional oil, methanol, ethanol, Dragendorff reagent, acetic acid anhydride, sulfuric acid, HCl, magnesium powder, FeCL3 1%, KLT plates, test tubes, dropper pipette, porcelain cup, filter paper, and a boiler. Results: Alkaloid, terpenoid and flavonoid compounds were found, and no polyphenol compound from phytochemistry screening and 16 constituents were identified from the GC-MS analysis, α-pinene was found as the major component (74.47%), delta-3-carene (9.62%), 1% of octane, dodecane, camphor, undecane, isodecane, sabinene, hexadecane, nonane, and <1% tridecane, cyclohexane, pentacosane, heptadecan, limonene, and camphene. Conclusion: Karo traditional oil works as a potent anti-inflammation at the inflammation phase of the wound healing process by suppressing the pro-inflammatory cytokines and promote anti-inflammatory cytokines.

Item Type:	Article
Uncontrolled Keywords:	Traditional medicine, Indonesia traditional medicine, Phytochemistry screening, Gas chromatography-mass spectrometry, Karo oil, Karo traditional oil, Wound healing.
Subjects:	R Medicine > R Medicine (General)
Divisions:	Jurnal > Fakultas Kedokteran
Depositing User:	NOVA PRIMADINA PRIMADINA
Date Deposited:	27 May 2022 08:48
Last Modified:	27 May 2022 08:48
URI:	http://repository.um-surabaya.ac.id/id/eprint/6187

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