Objective: The purpose of this research was to observe the bioactive compounds of Karo traditional oil by phytochemistry screening and gas
chromatography-mass spectrometry (GC-MS) analysis.
Method: We use 150 mg sample of traditional Karo traditional oil, methanol, ethanol, Dragendorff reagent, acetic acid anhydride, sulfuric acid, HCl,
magnesium powder, FeCL3 1%, KLT plates, test tubes, dropper pipette, porcelain cup, filter paper, and a boiler.
Results: Alkaloid, terpenoid and flavonoid compounds were found, and no polyphenol compound from phytochemistry screening and 16 constituents
were identified from the GC-MS analysis, α-pinene was found as the major component (74.47%), delta-3-carene (9.62%), 1% of octane, dodecane,
camphor, undecane, isodecane, sabinene, hexadecane, nonane, and <1% tridecane, cyclohexane, pentacosane, heptadecan, limonene, and camphene.
Conclusion: Karo traditional oil works as a potent anti-inflammation at the inflammation phase of the wound healing process by suppressing the
pro-inflammatory cytokines and promote anti-inflammatory cytokines.