Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD

Mosquito Culex quinquefasciatus is a vector of
nematode worms, namely Wuchereria bancrofti which is the
cause of filariasis in tropical and subtropical
countries. Distributed of Cx. quinquefasciatus is widely in
Indonesia with differences the geographical, resulting in the
adaptation to the environment and may results in a high
variation, both phenotypic (morphology) and genotypic
(genetic) variation. This study aims was to determine the
genetic diversity of mosquitoes Cx.quinquefasciatus as vector
filariasis in Pekalongan City and Regent. Genetic
characterization performed by PCR-RAPD using three primers,
ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using
UPGMA algorithm and Simple Matching Coefficient and
presented as dendrogram. The results showed a high genetic
diversity with the polymorphisms up to 100%.
Keywords: Culex quinquefasciatus, vector, filariasis, PCRRAPD