Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition

Ethnopharmacological relevance: Temu giring, the rhizome of Curcuma heyneana Valeton
& Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally
used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and
has many other bioactivities such as antioxidant, anticancer and antiinflammatory.
Aim of the study: The purpose of this study was to determine the antiaging activity of C.
heyneana to prove its traditional use.
Materials and methods: The antioxidant activity was determined using the DPPH free radical
method, and anti-aging activity was examined using in vitro assays such as tyrosinase
inhibitor and collagenase inhibitor. In vivo tests were performed by observing
histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid
contents and chromatographic profiles were determined by Thin Layer Chromatography
(TLC) - densitometry.
Results: In all in vitro assays, all of the extracts showed a dose-dependent manner in the final
concentration range from 62.5–500 µg/mL for DPPH assay and 31.25–250 µg/mL for
tyrosinase inhibition and collagenase inhibition assay. Curcuminoid (CUR), the active
principle of Curcuma genus shows antioxidant, tyrosinase inhibitor and collagenase inhibitor
activity greater than all C. heyneana extracts. The in vivo assay results showed that the
topical application of the crude extract of C. heyneana produced significant improvement
effects on the UV-induced skin structure damage. The total CUR content was correlated with
the anti-aging activity of Curcuma heyneana.
Conclusions: The results show that C. heyneana contains antioxidant compounds and has
potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as
a phyto-cosmeceutical